abc vectstain kit Search Results


vectstain elite abc kit  (Vector Laboratories)
95
Vector Laboratories vectstain elite abc kit
( A ) YM3711 was transfected to floating HEK293 cells using the FreeStyle 293 Expression System. After 4 day culture, the supernatant was harvested and filtered. Then, YM3711P were purified with an affinity column (HiTrap NHS-activated HP column) coupled with anti-Aβ1-42 antibodies. The eluate was evaluated by Western blotting with 6E10 mAb. ( B ) Binding assay. YM3711P at concentrations of 2 µg/ml (diamonds), 10 µg/ml (squares) and 20 µg/ml (triangles) or Aβ1-42 (2 µg/ml, circles) were coated onto microtiter wells. After blocking, biotinylated IgG purified from plasma of rabbits that had been vaccinated with YM3711 were applied and followed by HRP-labeled <t>VECTSTAIN</t> Elite <t>ABC</t> Kit. Samples showing O.D. more than 2.5 were further diluted and reexamined. Calculated O.D. values are shown in Panel B. ( C ) Competition assay. YM3711P at a concentration of 2 µg/ml was applied onto microtiter wells. Then, wells were incubated with a mixture of biotinylated anti-YM3711P IgG and unlabeled various competitors at 0.1 to 100 ratios. Competitors included 6E10 (diamonds), 4G8 (squares), anti-AβpE3-42 antibodies (triangles) and unlabeled anti-YM3711P IgG (circles). An asterisk indicates that higher concentration of the reagent was not available.
Vectstain Elite Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/abc+vectstain+kit/VECTASTAIN+ABC-AmP+Reagent+for+Western+Blot+Detection+(Standard+Kit)/pmc03785508-176-7-11
Average 95 stars, based on 1 article reviews
vectstain elite abc kit - by Bioz Stars, 2026-09
95/100 stars
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vectstain abc kit  (Vector Laboratories)
96
Vector Laboratories vectstain abc kit
( A ) YM3711 was transfected to floating HEK293 cells using the FreeStyle 293 Expression System. After 4 day culture, the supernatant was harvested and filtered. Then, YM3711P were purified with an affinity column (HiTrap NHS-activated HP column) coupled with anti-Aβ1-42 antibodies. The eluate was evaluated by Western blotting with 6E10 mAb. ( B ) Binding assay. YM3711P at concentrations of 2 µg/ml (diamonds), 10 µg/ml (squares) and 20 µg/ml (triangles) or Aβ1-42 (2 µg/ml, circles) were coated onto microtiter wells. After blocking, biotinylated IgG purified from plasma of rabbits that had been vaccinated with YM3711 were applied and followed by HRP-labeled <t>VECTSTAIN</t> Elite <t>ABC</t> Kit. Samples showing O.D. more than 2.5 were further diluted and reexamined. Calculated O.D. values are shown in Panel B. ( C ) Competition assay. YM3711P at a concentration of 2 µg/ml was applied onto microtiter wells. Then, wells were incubated with a mixture of biotinylated anti-YM3711P IgG and unlabeled various competitors at 0.1 to 100 ratios. Competitors included 6E10 (diamonds), 4G8 (squares), anti-AβpE3-42 antibodies (triangles) and unlabeled anti-YM3711P IgG (circles). An asterisk indicates that higher concentration of the reagent was not available.
Vectstain Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/abc+vectstain+kit/Avidin%2FBiotin+Blocking+Kit/pmc03940907-58-4-7
Average 96 stars, based on 1 article reviews
vectstain abc kit - by Bioz Stars, 2026-09
96/100 stars
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vectstain elite abc hrp kit  (Vector Laboratories)
96
Vector Laboratories vectstain elite abc hrp kit
( A ) YM3711 was transfected to floating HEK293 cells using the FreeStyle 293 Expression System. After 4 day culture, the supernatant was harvested and filtered. Then, YM3711P were purified with an affinity column (HiTrap NHS-activated HP column) coupled with anti-Aβ1-42 antibodies. The eluate was evaluated by Western blotting with 6E10 mAb. ( B ) Binding assay. YM3711P at concentrations of 2 µg/ml (diamonds), 10 µg/ml (squares) and 20 µg/ml (triangles) or Aβ1-42 (2 µg/ml, circles) were coated onto microtiter wells. After blocking, biotinylated IgG purified from plasma of rabbits that had been vaccinated with YM3711 were applied and followed by HRP-labeled <t>VECTSTAIN</t> Elite <t>ABC</t> Kit. Samples showing O.D. more than 2.5 were further diluted and reexamined. Calculated O.D. values are shown in Panel B. ( C ) Competition assay. YM3711P at a concentration of 2 µg/ml was applied onto microtiter wells. Then, wells were incubated with a mixture of biotinylated anti-YM3711P IgG and unlabeled various competitors at 0.1 to 100 ratios. Competitors included 6E10 (diamonds), 4G8 (squares), anti-AβpE3-42 antibodies (triangles) and unlabeled anti-YM3711P IgG (circles). An asterisk indicates that higher concentration of the reagent was not available.
Vectstain Elite Abc Hrp Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/abc+vectstain+kit/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Universal)/pmc11461145__djae120_supplementary_data-25-16-34
Average 96 stars, based on 1 article reviews
vectstain elite abc hrp kit - by Bioz Stars, 2026-09
96/100 stars
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abc vectstain kit  (Vector Laboratories)
94
Vector Laboratories abc vectstain kit
( A ) YM3711 was transfected to floating HEK293 cells using the FreeStyle 293 Expression System. After 4 day culture, the supernatant was harvested and filtered. Then, YM3711P were purified with an affinity column (HiTrap NHS-activated HP column) coupled with anti-Aβ1-42 antibodies. The eluate was evaluated by Western blotting with 6E10 mAb. ( B ) Binding assay. YM3711P at concentrations of 2 µg/ml (diamonds), 10 µg/ml (squares) and 20 µg/ml (triangles) or Aβ1-42 (2 µg/ml, circles) were coated onto microtiter wells. After blocking, biotinylated IgG purified from plasma of rabbits that had been vaccinated with YM3711 were applied and followed by HRP-labeled <t>VECTSTAIN</t> Elite <t>ABC</t> Kit. Samples showing O.D. more than 2.5 were further diluted and reexamined. Calculated O.D. values are shown in Panel B. ( C ) Competition assay. YM3711P at a concentration of 2 µg/ml was applied onto microtiter wells. Then, wells were incubated with a mixture of biotinylated anti-YM3711P IgG and unlabeled various competitors at 0.1 to 100 ratios. Competitors included 6E10 (diamonds), 4G8 (squares), anti-AβpE3-42 antibodies (triangles) and unlabeled anti-YM3711P IgG (circles). An asterisk indicates that higher concentration of the reagent was not available.
Abc Vectstain Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/abc+vectstain+kit/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Goat+IgG)/pmc03682301-80-1-7
Average 94 stars, based on 1 article reviews
abc vectstain kit - by Bioz Stars, 2026-09
94/100 stars
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vectstain abc ap kit  (Vector Laboratories)
93
Vector Laboratories vectstain abc ap kit
( A ) YM3711 was transfected to floating HEK293 cells using the FreeStyle 293 Expression System. After 4 day culture, the supernatant was harvested and filtered. Then, YM3711P were purified with an affinity column (HiTrap NHS-activated HP column) coupled with anti-Aβ1-42 antibodies. The eluate was evaluated by Western blotting with 6E10 mAb. ( B ) Binding assay. YM3711P at concentrations of 2 µg/ml (diamonds), 10 µg/ml (squares) and 20 µg/ml (triangles) or Aβ1-42 (2 µg/ml, circles) were coated onto microtiter wells. After blocking, biotinylated IgG purified from plasma of rabbits that had been vaccinated with YM3711 were applied and followed by HRP-labeled <t>VECTSTAIN</t> Elite <t>ABC</t> Kit. Samples showing O.D. more than 2.5 were further diluted and reexamined. Calculated O.D. values are shown in Panel B. ( C ) Competition assay. YM3711P at a concentration of 2 µg/ml was applied onto microtiter wells. Then, wells were incubated with a mixture of biotinylated anti-YM3711P IgG and unlabeled various competitors at 0.1 to 100 ratios. Competitors included 6E10 (diamonds), 4G8 (squares), anti-AβpE3-42 antibodies (triangles) and unlabeled anti-YM3711P IgG (circles). An asterisk indicates that higher concentration of the reagent was not available.
Vectstain Abc Ap Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/abc+vectstain+kit/VECTASTAIN+ABC-AP+Staining+KIT+(Alkaline+Phosphatase%2C+Rabbit+IgG)/pmc06701817-328-38-49
Average 93 stars, based on 1 article reviews
vectstain abc ap kit - by Bioz Stars, 2026-09
93/100 stars
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alkaline phosphatase abc kit  (Vector Laboratories)
95
Vector Laboratories alkaline phosphatase abc kit
( A ) YM3711 was transfected to floating HEK293 cells using the FreeStyle 293 Expression System. After 4 day culture, the supernatant was harvested and filtered. Then, YM3711P were purified with an affinity column (HiTrap NHS-activated HP column) coupled with anti-Aβ1-42 antibodies. The eluate was evaluated by Western blotting with 6E10 mAb. ( B ) Binding assay. YM3711P at concentrations of 2 µg/ml (diamonds), 10 µg/ml (squares) and 20 µg/ml (triangles) or Aβ1-42 (2 µg/ml, circles) were coated onto microtiter wells. After blocking, biotinylated IgG purified from plasma of rabbits that had been vaccinated with YM3711 were applied and followed by HRP-labeled <t>VECTSTAIN</t> Elite <t>ABC</t> Kit. Samples showing O.D. more than 2.5 were further diluted and reexamined. Calculated O.D. values are shown in Panel B. ( C ) Competition assay. YM3711P at a concentration of 2 µg/ml was applied onto microtiter wells. Then, wells were incubated with a mixture of biotinylated anti-YM3711P IgG and unlabeled various competitors at 0.1 to 100 ratios. Competitors included 6E10 (diamonds), 4G8 (squares), anti-AβpE3-42 antibodies (triangles) and unlabeled anti-YM3711P IgG (circles). An asterisk indicates that higher concentration of the reagent was not available.
Alkaline Phosphatase Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/abc+vectstain+kit/VECTASTAIN+ABC-AP+Staining+KIT+(Alkaline+Phosphatase%2C+Universal)/pmc04512711-98-19-24
Average 95 stars, based on 1 article reviews
alkaline phosphatase abc kit - by Bioz Stars, 2026-09
95/100 stars
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vectstain elite abc rat igg kit  (Vector Laboratories)
94
Vector Laboratories vectstain elite abc rat igg kit
( A ) YM3711 was transfected to floating HEK293 cells using the FreeStyle 293 Expression System. After 4 day culture, the supernatant was harvested and filtered. Then, YM3711P were purified with an affinity column (HiTrap NHS-activated HP column) coupled with anti-Aβ1-42 antibodies. The eluate was evaluated by Western blotting with 6E10 mAb. ( B ) Binding assay. YM3711P at concentrations of 2 µg/ml (diamonds), 10 µg/ml (squares) and 20 µg/ml (triangles) or Aβ1-42 (2 µg/ml, circles) were coated onto microtiter wells. After blocking, biotinylated IgG purified from plasma of rabbits that had been vaccinated with YM3711 were applied and followed by HRP-labeled <t>VECTSTAIN</t> Elite <t>ABC</t> Kit. Samples showing O.D. more than 2.5 were further diluted and reexamined. Calculated O.D. values are shown in Panel B. ( C ) Competition assay. YM3711P at a concentration of 2 µg/ml was applied onto microtiter wells. Then, wells were incubated with a mixture of biotinylated anti-YM3711P IgG and unlabeled various competitors at 0.1 to 100 ratios. Competitors included 6E10 (diamonds), 4G8 (squares), anti-AβpE3-42 antibodies (triangles) and unlabeled anti-YM3711P IgG (circles). An asterisk indicates that higher concentration of the reagent was not available.
Vectstain Elite Abc Rat Igg Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/abc+vectstain+kit/Rat+IgG+(Control+Antibody)/pm32596272-102-13-19
Average 94 stars, based on 1 article reviews
vectstain elite abc rat igg kit - by Bioz Stars, 2026-09
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vectstain abc standard kit  (Vector Laboratories)
96
Vector Laboratories vectstain abc standard kit
( A ) YM3711 was transfected to floating HEK293 cells using the FreeStyle 293 Expression System. After 4 day culture, the supernatant was harvested and filtered. Then, YM3711P were purified with an affinity column (HiTrap NHS-activated HP column) coupled with anti-Aβ1-42 antibodies. The eluate was evaluated by Western blotting with 6E10 mAb. ( B ) Binding assay. YM3711P at concentrations of 2 µg/ml (diamonds), 10 µg/ml (squares) and 20 µg/ml (triangles) or Aβ1-42 (2 µg/ml, circles) were coated onto microtiter wells. After blocking, biotinylated IgG purified from plasma of rabbits that had been vaccinated with YM3711 were applied and followed by HRP-labeled <t>VECTSTAIN</t> Elite <t>ABC</t> Kit. Samples showing O.D. more than 2.5 were further diluted and reexamined. Calculated O.D. values are shown in Panel B. ( C ) Competition assay. YM3711P at a concentration of 2 µg/ml was applied onto microtiter wells. Then, wells were incubated with a mixture of biotinylated anti-YM3711P IgG and unlabeled various competitors at 0.1 to 100 ratios. Competitors included 6E10 (diamonds), 4G8 (squares), anti-AβpE3-42 antibodies (triangles) and unlabeled anti-YM3711P IgG (circles). An asterisk indicates that higher concentration of the reagent was not available.
Vectstain Abc Standard Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/abc+vectstain+kit/Biotinylated+Rabbit+Anti-Goat+IgG+Antibody/pmc04326077-247-19-25
Average 96 stars, based on 1 article reviews
vectstain abc standard kit - by Bioz Stars, 2026-09
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vectstain elite abc kit  (Maravai LifeSciences)
90
Maravai LifeSciences vectstain elite abc kit
( A ) YM3711 was transfected to floating HEK293 cells using the FreeStyle 293 Expression System. After 4 day culture, the supernatant was harvested and filtered. Then, YM3711P were purified with an affinity column (HiTrap NHS-activated HP column) coupled with anti-Aβ1-42 antibodies. The eluate was evaluated by Western blotting with 6E10 mAb. ( B ) Binding assay. YM3711P at concentrations of 2 µg/ml (diamonds), 10 µg/ml (squares) and 20 µg/ml (triangles) or Aβ1-42 (2 µg/ml, circles) were coated onto microtiter wells. After blocking, biotinylated IgG purified from plasma of rabbits that had been vaccinated with YM3711 were applied and followed by HRP-labeled <t>VECTSTAIN</t> Elite <t>ABC</t> Kit. Samples showing O.D. more than 2.5 were further diluted and reexamined. Calculated O.D. values are shown in Panel B. ( C ) Competition assay. YM3711P at a concentration of 2 µg/ml was applied onto microtiter wells. Then, wells were incubated with a mixture of biotinylated anti-YM3711P IgG and unlabeled various competitors at 0.1 to 100 ratios. Competitors included 6E10 (diamonds), 4G8 (squares), anti-AβpE3-42 antibodies (triangles) and unlabeled anti-YM3711P IgG (circles). An asterisk indicates that higher concentration of the reagent was not available.
Vectstain Elite Abc Kit, supplied by Maravai LifeSciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/abc+vectstain+kit/vectastain+abc+kit/pmc11011967-198-25-33
Average 90 stars, based on 1 article reviews
vectstain elite abc kit - by Bioz Stars, 2026-09
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vectstain elite abc complex  (Vector Laboratories)
96
Vector Laboratories vectstain elite abc complex
( A ) YM3711 was transfected to floating HEK293 cells using the FreeStyle 293 Expression System. After 4 day culture, the supernatant was harvested and filtered. Then, YM3711P were purified with an affinity column (HiTrap NHS-activated HP column) coupled with anti-Aβ1-42 antibodies. The eluate was evaluated by Western blotting with 6E10 mAb. ( B ) Binding assay. YM3711P at concentrations of 2 µg/ml (diamonds), 10 µg/ml (squares) and 20 µg/ml (triangles) or Aβ1-42 (2 µg/ml, circles) were coated onto microtiter wells. After blocking, biotinylated IgG purified from plasma of rabbits that had been vaccinated with YM3711 were applied and followed by HRP-labeled <t>VECTSTAIN</t> Elite <t>ABC</t> Kit. Samples showing O.D. more than 2.5 were further diluted and reexamined. Calculated O.D. values are shown in Panel B. ( C ) Competition assay. YM3711P at a concentration of 2 µg/ml was applied onto microtiter wells. Then, wells were incubated with a mixture of biotinylated anti-YM3711P IgG and unlabeled various competitors at 0.1 to 100 ratios. Competitors included 6E10 (diamonds), 4G8 (squares), anti-AβpE3-42 antibodies (triangles) and unlabeled anti-YM3711P IgG (circles). An asterisk indicates that higher concentration of the reagent was not available.
Vectstain Elite Abc Complex, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/abc+vectstain+kit/VECTOR+NovaRED+Peroxidase+(HRP)+Substrate+Kit/pmc04622833-260-0-4
Average 96 stars, based on 1 article reviews
vectstain elite abc complex - by Bioz Stars, 2026-09
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( A ) YM3711 was transfected to floating HEK293 cells using the FreeStyle 293 Expression System. After 4 day culture, the supernatant was harvested and filtered. Then, YM3711P were purified with an affinity column (HiTrap NHS-activated HP column) coupled with anti-Aβ1-42 antibodies. The eluate was evaluated by Western blotting with 6E10 mAb. ( B ) Binding assay. YM3711P at concentrations of 2 µg/ml (diamonds), 10 µg/ml (squares) and 20 µg/ml (triangles) or Aβ1-42 (2 µg/ml, circles) were coated onto microtiter wells. After blocking, biotinylated IgG purified from plasma of rabbits that had been vaccinated with YM3711 were applied and followed by HRP-labeled VECTSTAIN Elite ABC Kit. Samples showing O.D. more than 2.5 were further diluted and reexamined. Calculated O.D. values are shown in Panel B. ( C ) Competition assay. YM3711P at a concentration of 2 µg/ml was applied onto microtiter wells. Then, wells were incubated with a mixture of biotinylated anti-YM3711P IgG and unlabeled various competitors at 0.1 to 100 ratios. Competitors included 6E10 (diamonds), 4G8 (squares), anti-AβpE3-42 antibodies (triangles) and unlabeled anti-YM3711P IgG (circles). An asterisk indicates that higher concentration of the reagent was not available.

Journal: PLoS ONE

Article Title: Development of a New DNA Vaccine for Alzheimer Disease Targeting a Wide Range of Aβ Species and Amyloidogenic Peptides

doi: 10.1371/journal.pone.0075203

Figure Lengend Snippet: ( A ) YM3711 was transfected to floating HEK293 cells using the FreeStyle 293 Expression System. After 4 day culture, the supernatant was harvested and filtered. Then, YM3711P were purified with an affinity column (HiTrap NHS-activated HP column) coupled with anti-Aβ1-42 antibodies. The eluate was evaluated by Western blotting with 6E10 mAb. ( B ) Binding assay. YM3711P at concentrations of 2 µg/ml (diamonds), 10 µg/ml (squares) and 20 µg/ml (triangles) or Aβ1-42 (2 µg/ml, circles) were coated onto microtiter wells. After blocking, biotinylated IgG purified from plasma of rabbits that had been vaccinated with YM3711 were applied and followed by HRP-labeled VECTSTAIN Elite ABC Kit. Samples showing O.D. more than 2.5 were further diluted and reexamined. Calculated O.D. values are shown in Panel B. ( C ) Competition assay. YM3711P at a concentration of 2 µg/ml was applied onto microtiter wells. Then, wells were incubated with a mixture of biotinylated anti-YM3711P IgG and unlabeled various competitors at 0.1 to 100 ratios. Competitors included 6E10 (diamonds), 4G8 (squares), anti-AβpE3-42 antibodies (triangles) and unlabeled anti-YM3711P IgG (circles). An asterisk indicates that higher concentration of the reagent was not available.

Article Snippet: Biotinylated anti-mouse IgG and horseradish peroxidase (HRP)-labeled VECTSTAIN Elite ABC Kit (Vector).

Techniques: Transfection, Expressing, Purification, Affinity Column, Western Blot, Binding Assay, Blocking Assay, Labeling, Competitive Binding Assay, Concentration Assay, Incubation

( A ) Paraffin-embedded brains sections of Tg mice were incubated with plasma taken from animals that had been immunized with YM3711. Samples were diluted 1∶100 to 1∶1,000. After washing, sections were incubated with cy3-conjugated or biotinylated secondary antibodies followed by HRP-labeled Vestastain Elite ABC kit. Intracellular Aβ immunoreactivities are indicated by arrows. Brain sections taken from five Tg mice were stained and the representative results are shown. ( B ) Immunoreactivity scores were graded into the following categories: absent immunoreactivity, (−); weak immunoreactivity, (+); moderate immunoreactivity, (++); and strong immunoreactivity, (+++).

Journal: PLoS ONE

Article Title: Development of a New DNA Vaccine for Alzheimer Disease Targeting a Wide Range of Aβ Species and Amyloidogenic Peptides

doi: 10.1371/journal.pone.0075203

Figure Lengend Snippet: ( A ) Paraffin-embedded brains sections of Tg mice were incubated with plasma taken from animals that had been immunized with YM3711. Samples were diluted 1∶100 to 1∶1,000. After washing, sections were incubated with cy3-conjugated or biotinylated secondary antibodies followed by HRP-labeled Vestastain Elite ABC kit. Intracellular Aβ immunoreactivities are indicated by arrows. Brain sections taken from five Tg mice were stained and the representative results are shown. ( B ) Immunoreactivity scores were graded into the following categories: absent immunoreactivity, (−); weak immunoreactivity, (+); moderate immunoreactivity, (++); and strong immunoreactivity, (+++).

Article Snippet: Biotinylated anti-mouse IgG and horseradish peroxidase (HRP)-labeled VECTSTAIN Elite ABC Kit (Vector).

Techniques: Incubation, Labeling, Staining